Cross-reactivity of a commercial chemiluminescence immunoassay for human chorionic gonadotropin with the free β-subunit
✍ Scribed by Luppa, P. ;Spöttl, G. ;Saller, B. ;Neumeier, D. ;Mann, K.
- Publisher
- John Wiley and Sons
- Year
- 1992
- Weight
- 423 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
An enhanced chemiluminescence immunoassay for the determination of serum human chorionic gonadotropin (HCG) in specimens f r o m oncology patients has been assessed with respect to i t s cross-reactivity with the free HCG @-subunit (HCG-$). The assay, standardized against the First International Reference Preparation 751537. had a crossreactivity with the free $-subunit o f 6 2 5 % (molar basis). Therefore this assay achieves high sensitivity for the detection of either intact HCG or free HCG-fl in serum of patients with seminomatous o r nonseminomatous testicular cancers. Results of both assays, the in-house immunoradiometric assay ( + HCG-@) and the Amerlite HCG-60 assay, showed a close correlation (R = 0.854-0.960) when serum samples f r o m tumour patients were analyzed. Moreover, the content o f free $-subunit determined in a specific HCG-$ assay, could be quantitatively measured in the enhanced chemiluminescence immunoassay. Thus, this assay is suitable for oncology use, b u t also highlights the limitations of measuring HCG in serum samples.