Cross-reactivity and antigenic structure of the C 5 component of human complement
β Scribed by K. Bauer
- Publisher
- Springer
- Year
- 1970
- Tongue
- English
- Weight
- 287 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0028-1042
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Human B and T lymphoblastoid cell lines were shown to synthesize C5. C5 synthesis was quantitated with an enzyme-linked immunosorbent assay (ELISA) that utilized a pool of C5-specific monoclonal antibodies (mAbs). Some level of C5 synthesis was detected in all eight of the B and T cell lines examine
Proteins were separated by prolonged isoelectric focusing in polyacrylamide gels, whereupon C2 bands were detected by a specific hemolytic assay. This was performed by treating the gel with iodine to increase C2 activity, and then developing C2 bands with an agarose gel overlay containing sensitized