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Cross-linking studies with the uvrA and uvrB proteins of E. coli

✍ Scribed by Peter Strike; W.Dean Rupp


Book ID
116108053
Publisher
Elsevier Science
Year
1985
Weight
883 KB
Volume
145
Category
Article
ISSN
0167-8817

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The use of sym-triazine trichloride in R
✍ Oste, Christian ;Brimacombe, Richard πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 578 KB

The reagent sym-triazine trichloride is used as a bifunctional reagent to generate RNA-protein cross-links within intact ribosomal subunits from E. coli. The reaction takes place in a stepwise manner, involving substitution of one chlorine atom at 12 degrees and pH 8, and substitution of the second