Cross-linking of surface IgM, but not surface IgD receptors, by soluble monoclonal antibodies primes murine B cells to secrete immunoglobulin in response to lymphokines
✍ Scribed by Catherine Phillips; Gerry G. B. Klaus
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 532 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Cross-linking of surface IgM, but not surface IgD receptors, by soluble monoclonal antibodies primes murine B cells to secrete immunoglobulin in response to lymphokines
We have previously reported the development of a two-step culture system in which soluble anti-p monoclonal antibodies prime small resting murine B cells to secrete immunoglobulin (Ig) in response to restimulation with a mixture of interleukin-4 (IL-4) and IL-5. Here we have extended these studies to investigate the effects of engaging surface IgD (sIgD). We find that, unlike anti-p, three different anti-6 monoclonal antibodies did not prime Bcells to secrete Ig. In addition, these anti-6 antibodies inhibited anti-p-stimulated priming for Ig secretion, while enhancing DNA synthesis in response to anti-p. Furthermore, anti4 antibodies still inhibited anti-p-induced priming when added 24-48 h after anti-p. These results therefore suggest that triggering of sIgD on B cells induces a dominant inhibitory signal which is not necessarily dependent upon co-ligation of sIgM and sIgD receptors. In addition, these findings raise the possibility that ligating sIgM or sIgD receptors on mature B cells in the absence of Tcell help, may produce different downstream effects.