Crop Breeding: Genetic Improvement Methods

✍ Scribed by Pasquale Tripodi

• Publisher: Springer US;Humana
• Year: 2021
• Tongue: English
• Leaves: 284
• Series: Methods in Molecular Biology 2264
• Edition: 1st ed.
• Category: Library

✦ Synopsis

This volume describes breeding methods for the development of biparental and multiparental mapping populations. Chapters detail lab protocols for high-throughput isolation of nucleic acids and metabolites, high performing genotyping approaches, mapping strategies for QTLs, mutation identifications, computational, bioinformatic pipelines, tissue culture-based and transformation methods for androgenesis, ploidy modification, and RNA interference. Additional chapters highlight recent developed genome editing protocols including CRISPR and TALEN methods and methodologies for in-field/in-soil plant phenotyping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Crop Breeding: Genetic Improvement Methodsaims to ensure successful results in the further study of this vital field.

✦ Table of Contents

Front Matter ....Pages i-xi
Methods of Development of Biparental Mapping Populations in Horticultural Crops (Pasquale Tripodi)....Pages 1-12
Multiparental Population in Crops: Methods of Development and Dissection of Genetic Traits (Isidore Diouf, Laura Pascual)....Pages 13-32
Using Metabolomics to Assist Plant Breeding (Saleh Alseekh, Alisdair R. Fernie)....Pages 33-46
High-Throughput DNA Isolation in Vegetable Crops for Genomics Applications (Pasquale Tripodi, Giovanna Festa)....Pages 47-53
High-Resolution Melting Analysis as a Tool for Plant Species Authentication (Liliana Grazina, Joana Costa, Joana S. Amaral, Isabel Mafra)....Pages 55-73
Specific-Locus Amplified Fragment Sequencing (SLAF-Seq) as High-Throughput SNP Genotyping Methods (Zhangsheng Zhu, Binmei Sun, Jianjun Lei)....Pages 75-87
Effective Mapping by Sequencing to Isolate Causal Mutations in the Tomato Genome (Fernando J. Yuste-Lisbona, José M. Jiménez-Gómez, Carmen Capel, Rafael Lozano)....Pages 89-103
Association Mapping in Plants (Pawan L. Kulwal, Ravinder Singh)....Pages 105-117
Practical Workflow from High-Throughput Genotyping to Genomic Estimated Breeding Values (GEBVs) (Felice Contaldi, Elisa Cappetta, Salvatore Esposito)....Pages 119-135
Guidelines for Setting Up a mRNA Sequencing Experiment and Best Practices for Bioinformatic Data Analysis (Teresa Rosa Galise, Salvatore Esposito, Nunzio D’Agostino)....Pages 137-162
RNA Interference (RNAi) in Tomato Crop Research (Pasquale Termolino)....Pages 163-175
Protoplast-Based Method for Genome Editing in Tetraploid Potato (Alessandro Nicolia, Ann-Sofie Fält, Per Hofvander, Mariette Andersson)....Pages 177-186
The Double-Layer Method to the Genesis of Androgenic Plants in Anemone coronaria (Andrea Copetta, Marina Laura)....Pages 187-196
Ploidy Modification for Plant Breeding Using In Vitro Organogenesis: A Case in Eggplant (Edgar García-Fortea, Ana García-Pérez, Esther Gimeno-Páez, Marina Martínez-López, Santiago Vilanova, Pietro Gramazio et al.)....Pages 197-206
Assembly of TALEN and mTALE-Act for Plant Genome Engineering (Aimee A. Malzahn, Yiping Qi)....Pages 207-218
Genome Editing to Achieve the Crop Ideotype in Tomato (Tomaš Čermák, Karla Gasparini, Zoltán Kevei, Agustin Zsögön)....Pages 219-244
Root System Phenotying of Soil-Grown Plants via RGB and Hyperspectral Imaging (Gernot Bodner, Mouhannad Alsalem, Alireza Nakhforoosh)....Pages 245-268
Light Drones for Basic In-Field Phenotyping and Precision Farming Applications: RGB Tools Based on Image Analysis (Federico Pallottino, Simone Figorilli, Cristina Cecchini, Corrado Costa)....Pages 269-278
Back Matter ....Pages 279-280

✦ Subjects

Biomedicine; Biomedical Engineering/Biotechnology; Agriculture