𝔖 Bobbio Scriptorium
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Creation of a Lipase Highly Selective for trans Fatty Acids by Protein Engineering

✍ Scribed by Dr. Henrike B. Brundiek; Dr. Andrew S. Evitt; Dr. Robert Kourist; Prof. Dr. Uwe T. Bornscheuer

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
564 KB
Volume
51
Category
Article
ISSN
0044-8249

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Protein-bound and unbound fatty acids can be efficiently separated at 0 degree C using a hydrophobic column-packing material (Lipidex 1000) similar to the separation of protein-bound and unbound steroids (E. Dahlberg, M. Snochowski, and J.-A. Gustafsson (1980) Anal Biochem. 106, 380-388). Protein-bo