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Cre-mediated recombination in cell lineages that express the progesterone receptor

✍ Scribed by Selma M. Soyal; Atish Mukherjee; Kevin Y.-S. Lee; Jie Li; Huaiguang Li; Francesco J. DeMayo; John P. Lydon

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
680 KB
Volume
41
Category
Article
ISSN
1526-954X

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✦ Synopsis

Using gene-targeting methods, a progesterone receptor Cre knockin (PR-Cre) mouse was generated in which Cre recombinase was inserted into exon 1 of the PR gene. The insertion positions the Cre gene downstream (and under the specific control) of the endogenous PR promoter. As for heterozygotes for the progesterone receptor knockout (PRKO) mutation, mice heterozygous for the Cre knockin insertion are phenotypically indistinguishable from wildtype. Crossing the PR-Cre with the ROSA26R reporter revealed that Cre excision activity is restricted to cells that express PR in progesterone-responsive tissues such as the uterus, ovary, oviduct, pituitary gland, and mammary gland. Initial characterization of the PR-Cre mouse underscores the utility of this model to precisely ablate floxed target genes specifically in cell lineages that express the PR. In the wider context of female reproductive tissue ontology, this model will be indispensable in tracing the developmental fate of cell lineages that descend from PR positive progenitors. genesis 41:58-66, 2005.

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