The peopling of the New World has been the focus of anthropological attention since the last century. Proponents of multiple migration models have claimed that patterns of variation among extant New World populations reflect ancient, discrete migrations to the Americas during the terminal Pleistocene. Although multiple migration models appear to explain patterns of both past and present craniometric variation, this interpretation rests on a number of key assumptions that require further investigation. We examined a series of Paleoindian (n Ο 11) and Archaic (n Ο 384) crania from North and South America, and compare these early samples to a large worldwide sample of late Holocene (n Ο 6,742) remains to assess within-and among-group variability in early samples, and to determine how patterns of variation could be viewed as a reflection of both population history and population structure. Analyses included univariate and multivariate analysis of variance, principal component analysis, calculation of biological distances, and multivariate allocation methods. We also performed model-bound analyses of these data, including Relethford-Blangero analysis and calculation of F ST . Our results indicate that under the assumptions of migration/founder models, the data are consistent with Paleoindians having derived from an undifferentiated Asian population that was not ancestral to modern American Indians. This view can be accommodated into existing models of multiple founders (migrations) in the New World. However, the assumptions required for such an interpretation are not realistic, and the diversity of early populations could as easily reflect population structuring processes such as genetic drift, demographic growth, and other phenomena. When the data were analyzed controlling for the effects of genetic drift (i.e., with smaller long-term effective population sizes for Paleoindians), the Paleoindian samples were no longer distinct from modern Native American populations. Other factors that need to be considered include processes involved in craniofacial change and adaptation during the past 10,000 years. Finally, patterns of variation in the North and South American Paleoindian samples are different, suggesting that the process of New World colonization is more complex than previously assumed.