Cover Picture: Two-Focus Fluorescence Correlation Spectroscopy: A New Tool for Accurate and Absolute Diffusion Measurements (ChemPhysChem 3/2007)

✍ Scribed by Thomas Dertinger; Victor Pacheco; Iris von der Hocht; Rudolf Hartmann; Ingo Gregor; Jörg Enderlein

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 32 KB
• Volume: 8
• Category: Article
• ISSN: 1439-4235
• DOI: 10.1002/cphc.200790004

✦ Synopsis

The cover picture shows the two focal regions of a two‐focus fluorescence correlation spectroscopy set‐up with molecules diffusing through these regions. The depicted curves are the fluorescence autocorrelation curves for the first and the second focus, respectively, as well as the fluorescence cross‐correlation between both foci. In their Article on page 433 Dertinger et al. introduce an external ruler into a fluorescence correlation measurement by having two focal regions with a fixed and well‐defined distance. This allows for absolute and precise determination of molecular diffusion.