Cover Picture: Differential Protein Assembly on Micropatterned Surfaces with Tailored Molecular and Surface Multivalency (ChemBioChem 9/2006)

✍ Scribed by Ramūnas Valiokas; Goran Klenkar; Ali Tinazli; Robert Tampé; Bo Liedberg; Jacob Piehler

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 31 KB
• Volume: 7
• Category: Article
• ISSN: 1439-4227
• DOI: 10.1002/cbic.200690027

✦ Synopsis

The cover picture shows surface‐plasmon images of a chelator density array on a gold surface, which was employed for differential protein assembly. These arrays were generated by piezo‐dispensing ethanolic thiol solutions into a hydrophobic grid obtained by microcontact printing of an eicosane thiol. By mixing an oligo(ethylene glycol)‐terminated matrix thiol with different concentrations of mono‐ and bivalent chelator thiols, the density of the chelator group was varied in different array elements. The combination of different molecular multivalencies with density‐dependent surface multivalencies yielded differential binding affinities of histidine‐tagged proteins to different array elements. Thus, selective elution with a competitor was achieved from array elements with low binding affinity, which could be subsequently refilled with a different protein. This strategy opens new perspectives for dissection and assembly of multiple protein complexes combined with real‐time, unlabeled detection. Further details can be found in the article by J. Piehler et al. on p. 1325 ff.