Covalent modification in aqueous solution of poly-γ-D-glutamic acid from bacillus licheniformis

✍ Scribed by Elizabeth C. King; William J. Watkins; A. John Blacker; Timothy D. H. Bugg

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 119 KB
• Volume: 36
• Category: Article
• ISSN: 0887-624X
• DOI: 10.1002/(sici)1099-0518(19980915)36:12<1995::aid-pola4>3.0.co;2-n

✦ Synopsis

Methods for the covalent modification in aqueous solution of poly-␥-Dglutamic acid from Bacillus licheniformis have been studied. Co-derivatization of a synthetic UV-absorbent amine and ethanolamine, using a water-soluble carbodi-imide coupling agent, yielded a water-soluble modified polymer. Derivatization of the polymer was accompanied by cleavage of the ␥-linked polypeptide backbone, and a reduction in molecular mass from 170 to 10 kDa. A procedure was developed for the removal of noncovalently bound ligands by treatment with 5 M CaCl 2 . The polymer sidechains also reacted in aqueous solution with p-nitrophenyl acetate to form covalent linkages.