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Covalent Immobilization of Proteins onto (Maleic Anhydride- alt -methyl Vinyl Ether) Copolymers: Enhanced Immobilization of Recombinant Proteins

✍ Scribed by Ladavière, Catherine; Delair, Thierry; Domard, Alain; Novelli-Rousseau, Armelle; Mandrand, Bernard; Mallet, François


Book ID
120493436
Publisher
American Chemical Society
Year
1998
Tongue
English
Weight
146 KB
Volume
9
Category
Article
ISSN
1043-1802

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Covalent immobilization of bovine serum
✍ C. Ladavière; T. Delair; A. Domard; C. Pichot; B. Mandrand 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 158 KB 👁 2 views

The covalent immobilization of bovin serum albumin (BSA) onto maleic anhydride-alt-methyl vinyl ether copolymers (MAMVE) was successfully achieved under aqueous conditions. The grafting reaction was shown to be controlled by attractive electrostatic interactions and so took place at a low salt conce

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The covalent grafting of biological molecules to copolymers of maleic anhydride and methyl vinyl ether (MAMVE) has been used in various applications in diagnostics. To tentatively elucidate the phenomena involved in the control of the immobilization of oligodeoxynucleotides and proteins, the physico