Lipase (EC 3.1.1.3) from Candida rugosa was immobilized with DEAE-Sephadex A50, Sephadex G50, Sephadex LH-20, Amberlite IRA94, and Amberlite XAD-7. The enzyme immobilized with DEAE-Sephadex A50 was found to be most effective for continuous hydrolysis of olive oil in isooctane. For the continuous rea
β¦ LIBER β¦
Covalent immobilization of lipase in organic solvents
β Scribed by Maj-Britt Stark; Krister Holmberg
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 573 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0006-3592
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## Abstract The catalytic activities of lipases derived from __Pseudomonas__ sp and pretreated with various organic solvents were investigated. The activity of the solventβpretreated lipase was greater than that of native lipase in both the esterification reaction in an organic medium and the hydro