## Abstract The effectiveness of attaching flavin adenine dinucleotide (FAD) via a C bridge to Teflonβbonded carbon black (CB), and the subsequent immobilization of glucose oxidase on the FADβmodified electrodes has been studied by cyclic voltammetry. When FAD alone is bound to the electrode, it un
Covalent immobilization of glucose oxidase onto graphitic electrodes
β Scribed by H.M. Wu; R. Olier; N. Jaffrezic-Renault; P. Clechet; A. Nyamsi; C. Martelet
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 436 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0013-4686
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β¦ Synopsis
Abatraet4raphtuz substrates (plates and carbon fibre 7 pm m diameter) have been used and characterlzed m order to obtain glucose senWve electrodes Preparation of such electrodes before enzymatic sensitization and after cleamng involves a specific electrochermcal prttreatment of oxidation at + 2 3 V/ see Further covalent unmobrhzation of glucose oxldase proceeds through a grafting between surface carboxyl group and a carbodumide reagent Performances of enzymatic electrodes such as thar stability up to two months make them well smted for further apphcatlon m the biomedical field Key words carbon fibre, graphite,
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## Abstract **Summary:** New polymer/silica gel hybrid supports were prepared by coating high surface area of silica gel with modified acrylonitrile copolymer. The concentrations of the modifying agent (NaOH) and the modified polymer were varied. GOD was covalently immobilized on these hybrid suppo
The kinetic properties of glucose oxidase (EC 1.1.3.4) which has been covalently immobilized to a rotating glassy carbon electrode surface have been investigated. Analysis of the rotation rate dependence of the hydrogen peroxide-derived current suggests that oxygen mass transport to the enzyme-elect