Covalent immobilisation of glucose oxidase on methacrylate copolymers for use in an amperometric glucose sensor

✍ Scribed by C.E. Hall; E.A.H. Hall

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 739 KB
• Volume: 281
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85026-g

✦ Synopsis

Glucose oxidase was covalently immobilised on the surface of copolymers of methyl and glycidyl methacrylates. The enzyme modified polymers were deposited from dichloromethane solutions onto platinum electrodes to form an amperometric glucose sensor based on the electrochemical measurement of hydrogen peroxide. The enzyme has been shown to retain its activity throughout the immobilisation and casting procedure, and the influence of various experimental variables have been explored for optimum analytical performance.