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Covalent binding of poly(ethylene glycol) (PEG) to the surface of red blood cells inhibits aggregation and reduces low shear blood viscosity

✍ Scribed by Armstrong, Jonathan K.; Meiselman, Herbert J.; Fisher, Timothy C.

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 29 KB
Volume: 56
Category: Article
ISSN: 0361-8609
DOI: 10.1002/(sici)1096-8652(199709)56:1<26::aid-ajh5>3.0.co;2-4

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✦ Synopsis

A simple method to coat human red blood cells (RBC) with PEG is described. Using a reactive derivative, monomethoxy-PEG (mPEG) was covalently attached to the surface of RBC in aqueous media under mild conditions. The PEG coating dramatically reduced aggregation and low shear viscosity of RBC resuspended in autologous plasma, and inhibited RBC agglutination by blood group-specific antibodies. Morphology and deformability of the PEG-treated cells were unaltered. The PEG coating of the RBC surface may be of significant benefit in the treatment of a variety of diseases characterized by vasoocclusion or impaired blood flow, e.g.,

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✍ Domagoj Sabolovic; Claude Sestier; Paulette Perrotin; Roger Guillet; Maurel Tefi 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 856 KB

Covalent binding of polyethylene glycol to the surface of red blood cells as detected and followed up by cell electrophoresis and rheological methods Cyanuric chloride activated polyethylene glycol (PEG)-5000 was covalently coupled to murine and human red blood cells (pegylated RBC). Our purpose was