✦ LIBER ✦
Covalent Attachment of Peptides to Membranes for Dot-Blot Analysis of Glycosylation Sites and Epitopes
✍ Scribed by B. Canas; Z. Dai; H. Lackland; R. Poretz; S. Stein
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 423 KB
- Volume
- 211
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Noncovalent binding of proteins to membranes is often employed for dot-blot analysis with various visualization techniques. These techniques are usually not applicable to peptide dot-blot analysis due to peptide wash-off during the staining procedure. As exemplified with a synthetic peptide and peptides produced by proteolysis of a protein, it is possible to achieve efficient covalent attachment to Immobilon-AV membranes. The utility of this membrane has been demonstrated with immunostaining and carbohydrate staining procedures. C 1993 Academic Press, Inc.