Coupling of membrane-bound and detergent-solubilized sodium- and potassium-activated ATPase to p-benzoquinone-treated microtiter plates

Directional immobilization of sodium- an

Directional immobilization of sodium- and potassium-activated ATPase to expose its cytoplasmic part to the liquid phase on microtiter plates by wheat germ agglutinin

✍ Hideaki Nagamune; Osamu Urayamaj; Yukichi Hara; Fusao Ota; Katsuhiko Hirota; Yas 📂 Article 📅 1989 🏛 Elsevier Science 🌐 English ⚖ 575 KB

A convenient method for highly efficient and directional immobilization of intact sodium- and potassium-activated ATPase (Na,K-ATPase) using wheat germ agglutinin linked on microtiter plates was developed. Wheat germ agglutinin, which bound tightly to the beta-subunit of Na,K-ATPase and had no effec