๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Coupled, continuous and discontinuous fluorometric assays for trehalase activity

โœ Scribed by Kathleen A. Killick

Book ID
102983265
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
604 KB
Volume
105
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

Continuous and discontinuous coupled fluorometric assays which couple trehalose hydrolysis to peroxidation of the fluorogenic compounds eugenol(4-allyl-2-methoxyphenol) or p-hydroxyphenylacetic acid using glucose oxidase (EC 1.1.3.4) and peroxidase (EC 1.11.1.7) as ancillary enzymes have been developed for the measurement of trehalase (cu,c/-trehalose-1-D-glucohydrolase, EC 3.2.1.28) activity from the cellular slime mold, Dictyostelium discoideum.

๐Ÿ“œ SIMILAR VOLUMES

A continuous coupled polarographic assay
A continuous coupled polarographic assay for trehalase
โœ Kathleen A. Killick ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 470 KB

A continuous, coupled polarographic assay, which couples trehalose hydrolysis to OZ consumption using glucose oxidase (EC 1.1.3.4) and catalase (EC 1.11.1.6) as ancillary enzymes has been developed for the measurement of trehalase (o-a'-trehalose 1-D-glucohydrolase, EC 3.2.1.28) activity. With this