✦ LIBER ✦

Coumestrol promotes proliferation and osteoblastic differentiation in rat bone marrow stromal cells

✍ Scribed by Xiao-tao Wu; Bin Wang; Ji-nan Wei

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 117 KB
Volume: 90B
Category: Article
ISSN: 1552-4973
DOI: 10.1002/jbm.b.31326

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Although the effects of coumestrol on osteoblasts and osteoclasts can be summarized as increasing the bone density and preventing bone resorption, direct and detailed effects of coumestrol on bone marrow stromal cells remain obscure. In the present study, the effects of coumestrol on proliferation and osteoblastic differentiation of rat bone marrow stromal cells (BMSCs) have been investigated; the regulative effect of coumestrol on BMSCs and skeletal system has also been discussed. The results showed that treatment with coumestrol increased cellular activities (analyzed by MTT assay), alkaline phosphatase (ALP), type I collagen and osteocalcin (OCN) activity as well as the protein and gene expression of OPG, gene expression ratio of OPG/RANKL and gene expression of estrogen receptor α(ERα). These results demonstrate that phytoestrogen coumestrol has a direct enhancing effect on the proliferation and osteogenic differentiation of bone marrow stromal cells, which would lead to stimulation of bone formation, and it can also protect the whole skeletal system by regulating OPG/RANKL expression, and these effects may be mediated by ERα. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009

📜 SIMILAR VOLUMES

Cholestane-3β,5α,6β-triol inhibits osteo

Cholestane-3β,5α,6β-triol inhibits osteoblastic differentiation and promotes apoptosis of rat bone marrow stromal cells

✍ Hongmei Liu; Lan Yuan; Shanjin Xu; Kui Wang; Tianlan Zhang 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 280 KB

## Abstract Converging lines of evidence suggest that oxidized lipids, long recognized as a risk factor in atherogenesis, also contribute to osteoporosis, but the underlying mechanism is not understood in detail. The effect of atherogenesis related factors including oxysterols on the differentiatio

Proliferation and differentiation of ost

Proliferation and differentiation of osteoblasts and adipocytes in rat bone marrow stromal cell cultures: Effects of dexamethasone and calcitriol

✍ Hassan Atmani; Daniel Chappard; Michel F. Basle 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 278 KB 👁 1 views

During bone loss, osteoblast population can be replaced by adipose tissue. This apparent reciprocal relationship between decreased bone density and increased fat formation can be explained by an imbalance in the production of bone-forming and fat-forming cells in the marrow cavity. Thus, osteoblast

Effect of dexamethasone withdrawal on os

Effect of dexamethasone withdrawal on osteoblastic differentiation of bone marrow stromal cells

✍ Ryan M. Porter; William R. Huckle; Aaron S. Goldstein 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 427 KB 👁 1 views

## Abstract Dexamethasone is capable of directing osteoblastic differentiation of bone marrow stromal cells (BMSCs) in vitro, but its effects are not lineage‐specific, and sustained exposure has been shown to down‐regulate collagen synthesis and induce maturation of an adipocyte subpopulation withi

Osteoprogenitor cell frequency in rat bo

Osteoprogenitor cell frequency in rat bone marrow stromal populations: Role for heterotypic cell–cell interactions in osteoblast differentiation

✍ Jane E. Aubin 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 269 KB 👁 2 views

Glucocorticoids, notably dexamethasone (Dex), have been reported to be a requirement for osteoprogenitor cell differentiation in young adult rat bone marrow stromal cell populations. We have reinvestigated the requirement for Dex and analyzed the frequency of osteoprogenitor cells present. Stromal c

Collagen type I hydrogel allows migratio

Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells

✍ Eric Hesse; Theresa E. Hefferan; James E. Tarara; Carl Haasper; Rupert Meller; C 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 153 KB

## Abstract Hydrogels are potentially useful for many purposes in regenerative medicine including drug and growth factor delivery, as single scaffold for bone repair or as a filler of pores of another biomaterial in which host mesenchymal progenitor cells can migrate in and differentiate into matri

Intravenous bone marrow stromal cell the

Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat

✍ Jieli Chen; Yi Li; Mark Katakowski; Xiaoguang Chen; Lei Wang; Dunyue Lu; Mei Lu; 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 391 KB

## Abstract The present study investigates the induction of neurogenesis, reduction of apoptosis, and promotion of basic fibroblast growth factor (bFGF) expression as possible mechanisms by which treatment of stroke with bone marrow stromal cells (MSCs) improves neurological functional recovery. Ad