Cotranslational Incorporation of a Structurally Diverse Series of Proline Analogues in an Escherichia coli Expression System

Stereoselective Incorporation of an Unsa

Stereoselective Incorporation of an Unsaturated Isoleucine Analogue into a Protein Expressed in E. coli

✍ Marissa L. Mock; Thierry Michon; Jan C. M. van Hest; David A. Tirrell 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 131 KB

## Abstract __The unsaturated amino acid 2‐amino‐3‐methyl‐4‐pentenoic acid (__E__‐Ile) was prepared in the form of its (2__S__,3__S__),(2__R__,3__R__) and (2__S__,3__R__),(2__R__,3__S__) stereoisomeric pairs. The translational activities of__ SS__‐__E__‐Ile and__ SR__‐__E__‐Ile were assessed in an_