Cost-effective colorimetric microtitre plate enzymatic assays for sucrose, glucose and fructose in sugarcane tissue extracts
✍ Scribed by Campbell, James A; Hansen, Ross W; Wilson, John R
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 158 KB
- Volume
- 79
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Quantiücation of sucrose, glucose and fructose is essential for developing a physiological understanding of growth and development of plant species. This is usually achieved by either HPLC or enzymatic assays for speciüc sugars. The need for reliable and cost-eþ ective assays of large numbers of samples led us to compare microtitre plate-adapted enzymatic assays for sucrose, glucose and fructose with current HPLC protocols. The enzymatic assay techniques were modiüed without loss of precision or accuracy when compared to the original protocols. Application of HPLC and the modiüed enzymatic assay to a range of sugarcane stem extracts found no diþ erence in assay sensitivity. The enzymatic assay had considerable advantages in terms of sample throughput and costeþ ectiveness.