Corticosteroids induce proliferation but do not influence TNF- or IL-1Β-induced ICAM-1 expression of human dermal microvascular endothelial cells in vitro
✍ Scribed by U. Hettmannsperger; S. Tenorio; C. E. Orfanos; M. Detmar
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 546 KB
- Volume
- 285
- Category
- Article
- ISSN
- 0340-3696
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✦ Synopsis
The effects of hydrocortisone, dexamethasone, betamethasone 17-valerate and clobetasol propionate at concentrations of 10-s-10-12 M on the proliferation of human dermal microvascular endothelial cells (HDMEC) were studied in vitro. In addition, confluent HDMEC were treated with TNF (1000 U/ml) or IL-lfl (1000 U/ml) alone or in combination with the corticosteroids (10-s M, 10-6M) for 24 h, and cytokine-induced expression of intercellular adhesion molecule-1 (ICAM-1) was assessed by immunocytochemistry. Controls were treated either with growth medium or with the corticosteroids alone. All tested corticosteroids stimulated HDMEC growth after 4 and 6 days of treatment in a dose-dependent manner, as assessed by aH-thymidine incorporation and the 4-methylumbelliferyl heptanoate (MUH) assay. The minimal effective concentration was 10-9M for hydrocortisone, 10-1~
for dexamethasone and betamethasone, and 10-12 M for clobetasol. In untreated and in corticosteroidtreated cultures, less than 5% of the cells expressed ICAM-1. TNF and IL-lfl were strong inducers of ICAM-1 expression on 74% and 82% of the cells, respectively. None of the tested corticosteroids significantly influenced cytokine-induced ICAM-1 expression, suggesting that the anti-inflammatory effects of corticosteroids are not related to ICAM-1 modulation on HDMEC.