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Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution

โœ Scribed by Loschberger, A.; Franke, C.; Krohne, G.; van de Linde, S.; Sauer, M.


Book ID
126234945
Publisher
The Company of Biologists Ltd
Year
2014
Tongue
English
Weight
722 KB
Volume
127
Category
Article
ISSN
0021-9533

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โœ Reinat Nevo; Peter Markiewicz; Ruti Kapon; Michael Elbaum; Ziv Reich ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 1022 KB

The lateral resolution attained by scanning force microscopy (SFM) on disordered membrane protein structures is often limited. We have imaged one such structure -the nuclear pore complex, on chemically fixed nuclear envelopes isolated from Xenopus oocytes, optimizing procedures to maximize lateral r