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Correlation of Adenine H2/H8 Resonances in Uniformly 13C Labeled RNAs by 2D HCCH-TOCSY: A New Tool for 1H Assignment

✍ Scribed by Marino, John P.; Prestegard, James H.; Crothers, Donald M.

Book ID
126979205
Publisher
American Chemical Society
Year
1994
Tongue
English
Weight
234 KB
Volume
116
Category
Article
ISSN
0002-7863

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We have prepared RNAs uniformly 13C-labeled in the ribose ring and with the H3',H4',H5'/H5" protons specifically replaced with deuterons (3',4',5'/5"-2H-13C, termed d4-13C ribose), which offers the advantage of spectral simplification without sacrifice of sensitivity for the remaining protons. A 3D