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Correlation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H

✍ Scribed by Itaya, Mitsuhiro ;Crouch, Robert J.

Publisher: Springer
Year: 1991
Tongue: English
Weight: 523 KB
Volume: 227
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00273934

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✦ Synopsis

The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].

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