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Correlation between substratum roughness and wettability, cell adhesion, and cell migration

✍ Scribed by Lampin, M. ;Warocquier-Cl�rout, R. ;Legris, C. ;Degrange, M. ;Sigot-Luizard, M. F.

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
940 KB
Volume
36
Category
Article
ISSN
0021-9304

No coin nor oath required. For personal study only.

✦ Synopsis

Cell adhesion and spreading of chick embryo vas-which annihilated roughness effect and restored hydrophilic cular and corneal explants grown on rough and smooth poly

properties. An organotypic culture assay was carried out in (methyl methacrylate) (PMMA) were analyzed to test the an attempt to relate the biocompatibility to substratum surcell response specificity to substratum surface properties. face state. Cell migration was calculated from the area of cell Different degrees of roughness were obtained by sand-layer. Cellular adhesion was determined by measuring the blasting PMMA with alumina grains. Hydrophilic and hy-kinetic of release of enzymatically dissociated cells. A slight drophobic components of the surface free energy (SFE) were roughness raised the migration area to an upper extent no calculated according to Good-van Oss's model. Contact matter which cell type. Enhancement of the cell adhesion angles were determined using a computerized angle meter.

potential was related to the degree of roughness and the The apolar component of the SFE, Ͳs LW , increased with a hydrophobicity.

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