Correlation between structure, retention and activity of cholic acid derived cis–trans isomeric bis-steroidal tetraoxanes
✍ Scribed by Sandra Šegan; Filip Andrić; Aleksandra Radoičić; Dejan Opsenica; Bogdan Šolaja; Mario Zlatović; Dušanka Milojković-Opsenica
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 334 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
Both quantitative structure–retention (QSRR) and quantitative structure–activity relationship (QSAR) studies have been performed to correlate the molecular characteristics of seven pairs of cis–trans isomeric bis‐steroidal tetraoxanes with their reversed‐phase thin‐layer chromatography (RPTLC) retention as well as with their antiproliferative activity. 2D and 3D molecular descriptors as whole molecule representations together with retention parameters as well as with biological activity data were subjected to the multivariate statistical analysis (principal component analysis – PCA and hierarchical cluster analysis – HCA) in order to determine the most influential factors governing the retention and activity against human cervix carcinoma (HeLa) and human malignant melanoma (Fem‐X) cell lines. Both QSRR and QSAR models were built by means of the partial least‐squares (PLS) statistical method. It was found that hydrogen bond donating (HBD), hydrogen bond accepting (HBAcc), hydrophilic surface percentage (%HS) and hydrophilic–lipophilic balance (HLB) exhibit the strongest influence on retention. The most prominent factors affecting antiproliferative activity of the investigated substances are those relating to the size and shape of a molecule such as: connectivity indices, refractivity (Ref), surface area (SA), molecular volume and weight, polarizability (Pol) and those regarding the ability of hydrogen bonding (HB).