Correction of the map location for the phr gene in Escherichia coli K12
โ Scribed by Aziz Sancar; Claud S. Rupert
- Book ID
- 113191257
- Publisher
- Elsevier Science
- Year
- 1978
- Tongue
- English
- Weight
- 264 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0027-5107
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The pepN gene has been cloned into the multicopy plasmid pBR322. The restriction map of the insert was established and the gene was localized. By comparison with the restriction map of the plasmid pJP30 bearing the ompF region, it has been possible to order the ompF, asnS, and pepN genes. The ompF a
A set of lambdadilv phage has been examined that carry overlapping segments of isoleucine-valine structural and regulatory genes derived from the ilv cluster at 83 min on the Escherichia coli K-12 chromosome. The ilv genes present in these phage, and their order, have been determined by transduction