Correction of molecular heterozygotes in the course of transformation

✍ Scribed by Bresler, S. E. ;Kreneva, R. A. ;Kushev, V. V.

• Book ID: 104749727
• Publisher: Springer
• Year: 1968
• Tongue: English
• Weight: 797 KB
• Volume: 102
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00385983

✦ Synopsis

In the course of this work a method of elonal analysis of transformed cells was developed. This involves growing of cells on nonselective plates, replication of colonies on selective agar to score for recombinants, homogenization of initial colonies and their analysis for pure or mixed progeny.

The main result of these experiments is the fact that pure clones are formed with a probability dependent on the specificity of the mutation involved. Proof is given that the pure clones are due to the repair of molecular heterozygotes formed during transformation.

Clonal analysis of double transformants gives an approach to the study of independent or simultaneous correction of molecular hers. Experiment shows in case of linked markers that simultaneous repair is overwhelming. When the distance between the markers becomes big enough we find a transition to independent correction of hets. The data are in general agreement with the results of EPI~RUSSI-TAYLOR on transformation of Pneumococcus.