Correction of glucose carbon recycling for the determination of ‘true’ hepatic glucose production rates by (1-13C1)glucose

Determination of 13C Enrichment at Speci

Determination of 13C Enrichment at Specific Positions of Plasma Glucose by Gas Chromatographic-Mass Spectrometric Analysis of the 1,2:5,6-Diisopropylidene-3-O-Acetyl-α-Furanosyl Derivative

✍ F. Rocchiccioli; M. Auclair; P.F. Bougneres 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 467 KB

To determine 13C enrichment at specific positions of glucose, plasma samples (50-100 microliters) were deproteinized using ethyl alcohol, centrifuged, and evaporated to dryness, and the residues were derivatized with acetone/H2SO4 (100/1, v/v). After acetylation, the 1,2:5,6-diisopropylidene-3-O-ace