Correction for quenching of samples from an oxygen flask combustion method for tritium analysis
✍ Scribed by Billy Baggett; Thomas L. Presson; Jack B. Presson; James C. Coffey
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 238 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Addition of water to the reaction mixture also eliminates this peak, suggesting that a reaction with water may displace one or more of the trimethylsilyl groups. This more labile component can be restored by retreatment with trimethylchlorosilane and hexamethyldisilazane. An explanation for these two peaks could be the formation of two different trimethylsilyl derivatives. Sweeley et al.
(3) reported that all the free hydroxyl groups of sugars were silylated and that the yield was virtually quantitative.
However, under the conditions used, the formation of a silazane bond with the C-6 amino group could occur. Such a bond may behave quite differently with respect to its formation and subsequent lability. It has also been found that the uridine derivative will react with the labile adenosine derivative to form a third peak (seen between uridine and adenosine in Fig. 1). The exact nature of this complex is at this time unknown.
Investigations are being extended to clarify these interactions, thus permitting quantitative analysis, and to derive techniques for the resolution of the minor nucleotides from natural products.