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Correction for Inner Filter Effects in Fluorescence Spectroscopy

✍ Scribed by Hans-Peter Lutz; Pier Luigi Luisi

Book ID
102252913
Publisher
John Wiley and Sons
Year
1983
Tongue
German
Weight
312 KB
Volume
66
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

A method is proposed for correcting experimental fluorescence readings for inner filter effects, i.e. the absorption of the exciting light and/or the absorption of the emitted radiation, which cause the non‐linearity between fluorescence intensity and fluorophor concentration. Basically the method consists of measuring the fluorescence intensity at two different points along the diagonal in the cell. Unlike similar methods proposed in the literature, the two points are corrected simultaneously for both absorption of excitation and of emission radiation without the necessity of reading the optical density of the solution, and with a very simple data elaboration.

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A graphical correction procedure for inn
A graphical correction procedure for inner filter effect in fluorescence quenching titrations
✍ Marina L. Mertens; Jeremias H.R. KΓ€gi πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 615 KB

Inner filter effects constitute a serious limitation for fluorescence quenching titrations with absorbing ligands. In most cases, the conventional methods employed for correction of such effects yield reliable results only at low total absorbance and, hence, titration is often restricted to relative