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Coordinate Binding Studies of the Substrate (Factor X) with the Cofactor (Factor VIII) in the Assembly of the Factor X Activating Complex on the Activated Platelet Surface†

✍ Scribed by Ahmad, Syed S.; Walsh, Peter N.

Book ID
115459445
Publisher
American Chemical Society
Year
2002
Tongue
English
Weight
256 KB
Volume
41
Category
Article
ISSN
0006-2960

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The modification of human factor X by 2-sulfo-N-succinimidyl acetate was investigated and shown to produce a factor X species which, when activated, has no activity toward factor VIII. Acylation of factor X (0.9 microM) was carried out in the presence of 1 mM calcium at different reagent concentrati