Cooperative ligand binding by ferrihemoglobin: An experimental artifact?

✍ Scribed by Alphonso C. I. Anusiem; John G. Beetlestone

• Book ID: 102764708
• Publisher: Wiley (John Wiley & Sons)
• Year: 1976
• Tongue: English
• Weight: 510 KB
• Volume: 15
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1976.360150216

✦ Synopsis

Abstract

We have carried out a detailed study of ligand binding of ferrihemoglobins under various conditions. Our results show that n varies with time and that this variation is paralleled by changes in the spectrum of methemoglobin. This suggests some form of structural perturbation. The time‐dependent value of n is discussed in terms of the observed spectral changes accompanying prolonged equilibration.