๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Coomassie brilliant blue staining of lipids on thin-layer plates

โœ Scribed by Kazuo Nakamura; Shizuo Handa

Book ID
102987152
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
988 KB
Volume
142
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

Coomassie brilliant blue staining of lipids on silica gel thin-layer chromatography plates is described. This stain proved to be useful for the wide-range detection of simple and complex lipids on thin-layer plates. It can stain several classes of lipids, including cholesterol, cholesterol esters, glycerides, phospholipids, ceramides, and neutral and acidic glycosphingolipids. It stains the spots evenly without a corrosive reagent, and is simple to use and suitable for storage. The visual detection limits of this stain for lipids were 0.05 to 0.5 microgram.

๐Ÿ“œ SIMILAR VOLUMES

Metachromasy of peripheral nerve collage
Metachromasy of peripheral nerve collagen on polyacrylamide gels stained with Coomassie brilliant blue R-250
โœ Steven Micko; William W. Schlaepfer ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 569 KB

Endoneurial collagen stains metachromatically with Coomassie brilliant blue R-250 (C.I. 42660) when peripheral nerve proteins are solubilized with urea and SDS and then subjected to SDS-polyacrylamide gel electrophoresis. The metachromasy is reproducible under different fixing and staining condition