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Conversion of the dimeric D-amino acid oxidase from Rhodotorula gracilis to a monomeric form. A rational mutagenesis approach

✍ Scribed by Luciano Piubelli; Laura Caldinelli; Gianluca Molla; Mirella S Pilone; Loredano Pollegioni

Book ID
117104490
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
264 KB
Volume
526
Category
Article
ISSN
0014-5793

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✍ Simona Butò; Loredano Pollegioni; Luigi D'Angiuro; Mirella S. Pilone 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 679 KB

This study reports on the development of a bioreactor for the production of a-keto acids from D,L-or D-amino acids using Rhodotorula gracilis o-amino acid oxidase. D-Amino acid oxidase was co-immobilized with catalase on Affi-Gel 10 matrix, and the reactor was operated as a continuous-stirred tank r