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Conversion of D-xylose into xylitol by xylose reductase fromCandida pelliculosacoupled with the oxidoreductase system of methanogen strain HU

✍ Scribed by V. Kitpreechavanich; M. Hayashi; N. Nishio; S. Nagai


Book ID
104635236
Publisher
Springer Netherlands
Year
1984
Tongue
English
Weight
346 KB
Volume
6
Category
Article
ISSN
0141-5492

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✦ Synopsis


A preliminary test for the enzymatic conversion of D-xylose into xyli-to1 by the intact cells of Candida pe,UicuLo.ba (xylose reductase) coupled with the intact cells of a for-mate-utilizing methanogen strain HU (hydrogenase and F42~-NADP oxidoreductase)

was conducted by using H2 as an electron donor of NADP+.

In the system, NADP(H) was well regenerated v& the methanogen cells and about 90% conversion of xylose to xylitol (ca. 8 g/l) could be achieved at 35OC and pH 7.5 after 24 h incubation.