✦ LIBER ✦
Convenient genotyping of six myostatin mutations causing double-muscling in cattle using a multiplex oligonucleotide ligation assay
✍ Scribed by L Karim; W Coppieters; L Grobet; M Georges; A Valentini
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 187 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0268-9146
No coin nor oath required. For personal study only.
✦ Synopsis
We herein describe a procedure that allows for simultaneous genotyping of six loss‐of‐function mutations in the bovine myostatin gene associated with the double‐muscling phenotype. The proposed method relies on a multiplex oligonucleotide ligation assay and detection of the fluorescently labelled products using automatic sequencers.