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Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging

โœ Scribed by Hoebe, R A; Van Oven, C H; Gadella, T W J; Dhonukshe, P B; Van Noorden, C J F; Manders, E M M

Book ID
109909719
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
452 KB
Volume
25
Category
Article
ISSN
1087-0156

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Controlled Light Exposure Microscopy (CL
Controlled Light Exposure Microscopy (CLEM) : New Imaging Approach Reduces Photobleaching and Phototoxicity
โœ R. A. Hoebe; C. H. Van Oven; T. W. J. Gadella Jr; P. B. Dhonukshe; C. J. F. Van ๐Ÿ“‚ Article ๐Ÿ“… 2007 ๐Ÿ› Wiley (John Wiley & Sons) โš– 841 KB

Photobleaching and phototoxicity are often the Achilles' heels of fluorescence live-cell imaging. Controlled Light Exposure Microscopy (CLEM) is a novel, simple imaging approach that reduces photobleaching and phototoxicity 2 to 10-fold without compromising image quality. The basic concept of CLEM i