Controlled Fluorescence Resonance Energy Transfer from Multiple Fluorophores in Layer-by-Layer Assemblies

✍ Scribed by Ki-Se Kim; Seong Il Yoo; Minsoo Kim; Byeong-Hyeok Sohn

• Book ID: 102483170
• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 421 KB
• Volume: 211
• Category: Article
• ISSN: 1022-1352
• DOI: 10.1002/macp.201000309

✦ Synopsis

Abstract

We demonstrate precise control of multiple FRET within LbL assemblies to produce simultaneous light emission with enhanced intensity from a single excitation with a blue wavelength. Three different fluorescent dyes were attached to neat polyelectrolytes and then incorporated into the LbL assembly. With designed deposition sequence, the layer of blue‐absorbing dyes was placed in the adjacent layer of green‐ and red‐emitting dyes, while the layers of emitting dyes were separated with each other beyond their Förster radius by inserting neat polyelectrolytes between them. The LbL assemblies not only allowed FRET from the absorbing dyes to the emitting dyes but also prohibited FRET between the emitting dyes to produce simultaneously enhanced green and red emission. magnified image