Transient gene expression was studied in isolated protoplasts of barley (Hordeum vulgare L. cv Himalaya) transformed by electroporation. Two plasmid constructions were used, both of which contained the gene coding for neomycin phosphotransferase II (nptII) as a reporter gene. In one plasmid the repo
Control of transcription of α-amylase and rRNA genes in barley aleurone protoplasts by gibberellin and abscisic acid
✍ Scribed by Jacobsen, J. V.; Beach, L. R.
- Book ID
- 109739674
- Publisher
- Nature Publishing Group
- Year
- 1985
- Tongue
- English
- Weight
- 339 KB
- Volume
- 316
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/316275a0
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The plant hormone abscisic acid (ABA) mediates gene expression in barley aleurone protoplasts. In order to elucidate the essential functional groups of the ABA molecule, the specificity of a number of ABA analogues for inducing ABAregulated gene (e.g., RAB, BASI) expression in barley aleurone protop
When barley aleurone layers were incubated with (3)H-Gibberellin A1 ((3)H-GA1), the hormone was converted to (3)H-GA-X (not identified), (3)H-GA8 and two other compounds tentatively identified as (3)H-GA1-glucoside, and (3)H-GA8-glucoside. Uptake and metabolism of the (3)H-GA1 were markedly enhanced
Changes in bifunctional alpha-amylase/subtilisin inhibitor (BASI) expression induced by abscisic acid (ABA) were studied using in vitro cultured barley (Hordeum vulgare cv. Bonanza) embryos. The steady-state levels of BASI mRNA and BASI protein were increased by exogenously applied ABA. Accumulation