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Control of the Myc-Max mediated transactivation in yeast by natural promoter elements

✍ Scribed by Frank Hänel; Karen Peukert; Thomas Munder

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
538 KB
Volume
37
Category
Article
ISSN
0233-111X

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✦ Synopsis

Transcriptional activation studies involving the human oncoprotein and transcription factor Myc and its helix-loop-helix partner protein Max in mammalian cells are critical due to the presence of endogenous Myc and Max proteins. Here we show that co-expression of the human c-myc and max genes from 2p circle derived high copy number vectors in yeast cells stimulate the transcriptional activation of a LacZ reporter gene fused to the yeast cytochrome-c, oxidase minimal promoter containing the adenovirus major late promoter element (AMLPE). The exchange of the single Myc binding site in the AMLPE by the two E-box DNA motifs (CACGTG) present in the Myc responsive element of a human Myc target gene (omithine decarboxylase) in front of a promoter-reporter gene cassette results in a two-fold enhanced /3-galactosidase expression. Low expression of mux and high level expression of c-myc at the same time led to a further enhancement of transcriptional activation from this promoter-reporter gene cassette.

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