Control of the argECBH cluster in Escherichia coli
โ Scribed by Jacoby, George A.
- Publisher
- Springer
- Year
- 1972
- Tongue
- English
- Weight
- 887 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0026-8925
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๐ SIMILAR VOLUMES
Ribosomal RNA synthesis has been investigated in an in vitro system consisting of purified E. coli RNA polymerase and phi 80d3 DNA carrying rrnC operon. rRNA comprises about 25% of the total RNA synthesized in this system under optimal conditions and is stimulated by a crude protein fraction prepare
Nucleoids were isolated from Escherichia coli B/r cells in steady-stage growth at different rates. The number of RNA chains growing on each nucleoid was estimated from the amount and size of RNAs synthesized by endogenous RNA polymerase. This figure represents the number of RNA polymerase molecules
The effect of amino acid-starvation on the transcription in vitro of overall RNA and ribosomal RNA was investigated using nucleoids prepared from the exponentially growing and the amino acid-starved cells of rel+ and rel- strains of Escherichia coli. In this system, the synthesis of RNA is exclusive