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Control of normal differentiation of myeloid leukemic cells. VI. Inhibition of cell multiplication and the formation of macrophages

✍ Scribed by Joseph Lotem; Leo Sachs

Publisher
John Wiley and Sons
Year
1975
Tongue
English
Weight
541 KB
Volume
85
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

D^+^ but not D^−^ myeloid leukemic cells can be induced by the appropriate conditioned medium or by serum from endotoxin treated mice, to undergo cell migration in agar, cell attachment to the surface of a Petri dish and differentiation to mature macrophages and granulocytes. Inhibition of cell multiplication by cytosine arabinoside, hydroxyurea, mitomycin C, thymidine, 5‐bromodeoxyuridine, 5‐iododeoxyuridine, 5‐fluorodeoxyuridine or actinomycin D, but not by vinblastine or cycloheximide, induced cell migration, cell attachment to the Petri dish and the formation of macrophages in D^+^ cells. There was no induction of cell migration or formation of macrophages and a much lower induction of cell attachment in D^−^ cells. The induction of these changes in D^+^ cells required protein synthesis and the inhibitors showed the same toxicity for D^+^ and D^−^ cells. The results indicate, that the inhibitors induced specific surface membrane changes in D^+^ but not in D^−^ cells.

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