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Control of growth and recombinant protein synthesis by heat-shock in a mutant mammalian cell line

โœ Scribed by Alison Hovey; Chris R. Bebbington; Nigel Jenkins

Book ID
104635536
Publisher
Springer Netherlands
Year
1994
Tongue
English
Weight
400 KB
Volume
16
Category
Article
ISSN
0141-5492

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โœฆ Synopsis

The effect of heat-shock on cell growth and the induction of recombinant protein synthesis by a temperature-sensitive (ts) Chinese hamster ovary (CHO). cell line was investigated. An optimal regime of successive 2 hour heat-shocks (42ยฐC) over 72 hours was found to simultaneously arrest cell growth and induce the synthesis of recombinant protein. The enhanced induction achieved from growth arrested cells may find application in the production of cytotoxic proteins.

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