Control of granulopoiesis in man. III. Inhibition of colony formation by dense leukocytes
✍ Scribed by F. L. Baker; H. E. Broxmeyer; P. R. Galbraith
- Publisher
- John Wiley and Sons
- Year
- 1975
- Tongue
- English
- Weight
- 462 KB
- Volume
- 86
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Cellular feeder layers, prepared from normal blood leukocytes, usually stimulate human marrow to form colonies. A significant increase in the stimulating activity of unseparated leukocyte feeder layers is brought about following the removal of dense leukocytes in a manner which avoids enrichment of any remaining cell type. Restoration of dense leukocytes to a dense leukocyte depleted leukocyte feeder layer results in the reduction of stimulating activity to that of an unseparated leukocyte feeder; however, addition of dense leukocytes to unseparated leukocyte feeder layers has no effect on the stimulatory activity, over the range of concentrations used in this study.