Control of clubroot of crucifers by Phoma glomerata and its product epoxydon

✍ Scribed by Arie, Tsutomu; Kobayashi, Yumiko; Kono, Yoshiki; Gen, Okada; Yamaguchi, Isamu

• Book ID: 101215553
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 81 KB
• Volume: 55
• Category: Article
• ISSN: 1526-498X
• DOI: 10.1002/(sici)1096-9063(199905)55:5<602::aid-ps964>3.0.co;2-c

✦ Synopsis

library and identi®ed another cytochrome P450 cDNA from C quinquefasciatus larvae.

The PCR products of c250 bp were ampli®ed, and 35 and 29 clones from permethrin-suseceptible and -resistant JPal-per strains, respectively, were sequenced. Alignments of the deduced amino acid sequences from these clones revealed that there were seven distinct sequences. One of these clones, which accounted for more than 57% of the total clones, was used as a probe to re-screen the cDNA library. Another full-length cDNA was cloned and designated CYP6F1. Northern blot analysis revealed that the CYP6F1 gene in JPal-per strain appeared to be expressed more strongly than that in the S strain suggesting a possible involvement of this gene in resistance to pyrethroids. This result supports our previous ®nding that the content of cytochrome P450 was about 2.7 times higher in JPal-per strain than in the S strain. 1 Results of Southern blotting indicated that CYP6F1 genes from susceptible and JPal-per strains contain different Eco RI and Eco RV digestion sites, suggesting that these two strains have different genome structures.