## Abstract The kinetics of renaturation of bovine carbonic anhydrase II (CAII) were studied from 4ยฐ to 36ยฐ, at the relatively high [CAII] of 4 mg/mL. Following dilution to 1 M guanidinium chloride, aggregate formation is very rapid and reduces the formation of active enzyme. The CAII activity yiel
Control of aggregation in protein refolding: Cooperative effects of artificial chaperone and cold temperature
โ Scribed by Razieh Yazdanparast; Mohammad Ali Esmaeili; Fariba Khodagholi
- Book ID
- 116541759
- Publisher
- Elsevier Science
- Year
- 2007
- Tongue
- English
- Weight
- 865 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0141-8130
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## Abstract The denaturation and renaturation of carbonic anhydrase II (CAII) has been studied in several laboratories. Both thermodynamic and kinetic evidence support the existence of at least two intermediates between denatured and native protein. Previous studies have shown that on rapid dilutio
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