Control by phospho-adenosinediphospho-ribose of NADP-dependent isocitrate dehydrogenase and 6-phosphogluconate dehydrogenase in Streptomyces griseus
✍ Scribed by Dr. U. Gräfe; E. J. Bormann; G. Truckenbrodt
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 315 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
The metabolic function of NAD(P)‐glycohydrolase in the streptomycyin‐producing Streptomyces griseus was investigated. Phospho‐adenosinediphospho‐ribose, the product of NAD(P)‐glycohydrolase reaction was shown to interfere as a competitive inhibitor not only with the glucose‐6‐phosphate dehydrogenase (Voronina et al. 1978) but also with the NADP‐dependent isocitrate and 6‐phospho‐gluconate dehydrogenases. Inhibition kinetics were studied with isocitrate dehydrogenase from pig heart and 6‐phosphogluconate dehydrogenase from yeast as well as with mycelial extracts of a mutant of S. griseus lacking NAD(P)‐glycohydrolase.